filter_transcripts {BANDITS} | R Documentation

## Filter lowly abundant transcripts.

### Description

`filter_transcripts` filters transcripts, before loading the data, according to estimated transcript level counts. The function outputs a vector containing the list of transcripts which respect the filtering criteria across all samples (i.e., min_transcript_proportion, min_transcript_counts and min_gene_counts).

### Usage

    filter_transcripts(gene_to_transcript, transcript_counts,
      min_transcript_proportion = 0.01, min_transcript_counts = 1,
      min_gene_counts = 10)

### Arguments

`gene_to_transcript` | a matrix or data.frame with a list of gene-to-transcript correspondances. The first column represents the gene id, while the second one contains the transcript id.
`transcript_counts` | a matrix or data.frame, with 1 column per sample and 1 row per transcript, containing the estimated abundances for each transcript in each sample.
`min_transcript_proportion` | the minimum relative abundance (i.e., proportion) of a transcript in a gene.
`min_transcript_counts` | the minimum overall abundance of a transcript (adding counts from all samples).
`min_gene_counts` | the minimum overall abundance of a gene (adding counts from all samples).

### Details

Transcript pre-filtering is highly suggested: it both improves the performance of the method and decreases its computational cost.

### Value

A vector containing the list of transcripts which respect the filtering criteria.

### Author(s)

Simone Tiberi simone.tiberi@uzh.ch

### See Also

`filter_genes`, `create_data`, `BANDITS_data`

### Examples

    # specify the directory of the internal data:
    data_dir = system.file("extdata", package = "BANDITS")

    # load gene_to_transcript matching:
    data("gene_tr_id", package = "BANDITS")

    # Load the transcript level estimated counts via tximport:
    library(tximport)
    quant_files = file.path(data_dir, "STAR-salmon", paste0("sample", seq_len(4)), "quant.sf")
    txi = tximport(files = quant_files, type = "salmon", txOut = TRUE)
    counts = txi$counts

    # transcript pre-filtering:
    transcripts_to_keep = filter_transcripts(gene_to_transcript = gene_tr_id,
                                             transcript_counts = counts,
                                             min_transcript_proportion = 0.01,
                                             min_transcript_counts = 10,
                                             min_gene_counts = 20)
    head(transcripts_to_keep)

[Package BANDITS version 1.10.0 Index]