.singlePrepUMI4C {UMI4Cats} | R Documentation

## Prepar fastq files at a given barcode.

### Description

Prepar fastq files at a given barcode.

### Usage

    .singlePrepUMI4C(
      fq_R1,
      fq_R2,
      bait_seq,
      bait_pad,
      res_enz,
      prep_dir,
      numb_reads = 1e+09
    )

### Arguments

`fq_R1` | Fastq file R1.
`fq_R2` | Fastq file R2.
`bait_seq` | Character containing the bait primer sequence.
`bait_pad` | Character containing the pad sequence (sequence between the bait primer and the restriction enzyme sequence).
`res_enz` | Character containing the restriction enzyme sequence.
`prep_dir` | Prep directory.
`numb_reads` | Number of lines from the FastQ file to load in each loop. If having memory size problems, change it to a smaller number. Default=1e9.

### Value

Creates a compressed FASTQ file in `wk_dir/prep` named `basename(Fastq)).fq.gz`, containing the filtered reads with the UMI sequence in the header. A data.frame object with the statisitics is also returned.

[Package UMI4Cats version 1.4.0 Index]