subsetSummary {chipseq} | R Documentation

## Compute summaries for cumulative subsets of a short-read data set.

### Description

THIS FUNCTION IS DEFUNCT!

Divides a short-read dataset into several subsets, and computes various summaries cumulatively. The goal is to study the characteristics of the data as a function of sample size.

### Usage

    subsetSummary(x, chr, nstep, props = seq(0.1, 1, 0.1),
                  chromlens = seqlengths(x), fg.cutoff = 6, seqLen = 200,
                  fdr.cutoff = 0.001, use.fdr = FALSE, resample = TRUE,
                  islands = TRUE, verbose = getOption("verbose"))

### Arguments

`x` | A `"GRanges"` object representing alignment locations at the sample level.
`chr` | The chromosome for which the summaries are to be obtained. Must specify a valid element of `x`
`nstep` | The number of maps in each increment for the full dataset (not per-chromosome). This will be translated to a per-chromosome number proportionally.
`props` | Alternatively, an increasing sequence of proportions determining the size of each subset. Overrides `nstep`.
`chromlens` | A named vector of per-chromosome lengths, typically the result of `seqlengths`.
`fg.cutoff` | The coverage depth above which a region would be considered foreground.
`seqLen` | The number of bases to which to extend each read before computing coverage.
`resample` | Logical; whether to randomly reorder the reads before dividing them up into subsets. Useful to remove potential order effects (for example, if data from two lanes were combined to produce `x`).
`fdr.cutoff` | The maximum false discovery rate for a region that is considered to be foreground.
`use.fdr` | Whether to use the FDR detected peaks when calling foreground and background.
`islands` | Logical. If `TRUE`, the whole island would be considered foreground if the maximum depth equals or exceeds `fg.cutoff`. If `FALSE`, only the region above the cutoff would be considered foreground.
`verbose` | logical controlling whether progress information will be shown during computation (which is potentially long-running).

### Value

A data frame with various per-subset summaries.

### Note

This function should be considered preliminary, in that it might change significantly or simply be removed in a subsequent version. If you like it the way it is, please notify the maintainer.

### Author(s)

Deepayan Sarkar, Michael Lawrence

[Package chipseq version 1.44.0 Index]