batchTallies {h5vc} | R Documentation

## Tallying bam files in parallel using `BatchJobs` on high performance compute clusters (HPC)

### Description

These function tally a set of bam files in blocks spanning a specified region and write the results to an HDF5 tally file; uses `BatchJobs` for parallel computation on HPCs

### Usage

    batchTallyParam(
      bamFiles,
      destination,
      group,
      chrom, start, stop,
      blocksize = 100000,
      registryDir = tempdir(),
      resources = list("queue" = "research-rh6", "memory"="4000", "ncpus"="4", walltime="90:00"),
      q=25, ncycles = 0, max.depth=1000000,
      reference = NULL,
      sleep = 5
    )

    batchTallies( confList = batchTallyParam() )

    rerunBatchTallies( confList, tryCollect = TRUE )

    collectTallies(blocks, confList, registries )

### Arguments

`bamFiles` | A character vector of filenames of the bam files that should be tallies. Note that for writing to an HDF5 file the order of this vector must match the order of the Column field in the sampledata object that corresponds to the dataset - see `setSampleData` for details.
`reference` | A DNAString object containing the reference sequence corresponding to the region that is to be tallied – if this is `NULL` a consensus vote will be used to estimate the reference at any given position, this means you cannot detect variants with AF >= 0.5 anymore – especially when tallying more than one bamFile you really should specify this
`destination` | Filename of the HDF5 tally file that will be written to – this needs to contain all the groups and datasets already – see `prepareTallyFile` for details
`group` | Location within the tally file where the data will be written – e.g. `"/ExampleStudy/22"`
`chrom` | Chromosome in which to tally
`start` | First position of the tally
`stop` | Last position of the tally
`q` | quality cut-off for considering a base call
`ncycles` | number of sequencing cycles form the front and back of the read that should be considered unreliable - used for stratifying the nucleotide counts
`max.depth` | only tally a position if there are less than this many reads overlapping it - can prevent long runtimes in unreliable regions
`blocksize` | Size of the blocks in bases that the tallying will be performed in, this influences the number of jobs send to the cluster
`registryDir` | Directory in which the registries created by `BatchJobs` wil be held, this can be temporary since we delete them when we are done
`resources` | A named list specifying the resource requirements of the cluster jobs, this must contain names for the fields specified in the cluster configuration file – see the documentation of `BatchJobs` for details
`confList` | A configuration list as returned by a call to `batchTallyParam()`
`sleep` | Number of seconds to sleep before checking if blocks are finshed, increase this if you have large blocks and find the output of `batchTallies` to verbose
`tryCollect` | Boolean flag specifying whether the `rerunBatchTallies` function should try to collect data from the specified registries before re-submitting.
`blocks` | `data.frame` defining blocks to tally in, result of a cal to `defineBlocks`
`registries` | A list mapping registry IDs to the work paths of the corresponding registries

### Details

This is a wrapper function for applying `tallyBAM` to a set of bam files specified in the `bamFiles` argument. The order or samples along the sample dimension is the same as the order of the file names (i.e. the order of the `bamfiles` argument). The function uses `BatchJobs` to dispatch tallying in blocks along the genome to a HPC and collects the results and writes them into the HDF5 tally file specified in the `destination` parameter.

rerunBatchTallies can be used to re-submit failed blocks.

collectTallies can be used to manually collect tally data from the registries created by batchTallies

### Value

[None] – prints progress messages along the way.

### Author(s)

Paul Pyl

### Examples

    ## Not run:
    library(h5vc)
    files <- c("NRAS.AML.bam","NRAS.Control.bam")
    bamFiles <- file.path( system.file("extdata", package = "h5vcData"), files)
    chrom = "1"
    startpos <- 115247090
    endpos <- 115259515
    batchTallies( batchTallyParam(bamFiles, chrom, startpos, endpos) )

    ## End(Not run)

[Package h5vc version 2.28.0 Index]