plot_genes_branched_heatmap {monocle} | R Documentation

## Create a heatmap to demonstrate the bifurcation of gene expression along two branchs @description returns a heatmap that shows changes in both lineages at the same time. It also requires that you choose a branch point to inspect. Columns are points in pseudotime, rows are genes, and the beginning of pseudotime is in the middle of the heatmap. As you read from the middle of the heatmap to the right, you are following one lineage through pseudotime. As you read left, the other. The genes are clustered hierarchically, so you can visualize modules of genes that have similar lineage-dependent expression patterns.

### Description

Create a heatmap to demonstrate the bifurcation of gene expression along two branchs

@description returns a heatmap that shows changes in both lineages at the same time. It also requires that you choose a branch point to inspect. Columns are points in pseudotime, rows are genes, and the beginning of pseudotime is in the middle of the heatmap. As you read from the middle of the heatmap to the right, you are following one lineage through pseudotime. As you read left, the other. The genes are clustered hierarchically, so you can visualize modules of genes that have similar lineage-dependent expression patterns.

### Usage

    plot_genes_branched_heatmap(
      cds_subset,
      branch_point = 1,
      branch_states = NULL,
      branch_labels = c("Cell fate 1", "Cell fate 2"),
      cluster_rows = TRUE,
      hclust_method = "ward.D2",
      num_clusters = 6,
      hmcols = NULL,
      branch_colors = c("#979797", "#F05662", "#7990C8"),
      add_annotation_row = NULL,
      add_annotation_col = NULL,
      show_rownames = FALSE,
      use_gene_short_name = TRUE,
      scale_max = 3,
      scale_min = -3,
      norm_method = c("log", "vstExprs"),
      trend_formula = "~sm.ns(Pseudotime, df=3) * Branch",
      return_heatmap = FALSE,
      cores = 1,
      ...
    )

### Arguments

`cds_subset` | CellDataSet for the experiment (normally only the branching genes detected with branchTest)
`branch_point` | The ID of the branch point to visualize. Can only be used when reduceDimension is called with method = "DDRTree".
`branch_states` | The two states to compare in the heatmap. Mutually exclusive with branch_point.
`branch_labels` | The labels for the branchs.
`cluster_rows` | Whether to cluster the rows of the heatmap.
`hclust_method` | The method used by pheatmap to perform hirearchical clustering of the rows.
`num_clusters` | Number of clusters for the heatmap of branch genes
`hmcols` | The color scheme for drawing the heatmap.
`branch_colors` | The colors used in the annotation strip indicating the pre- and post-branch cells.
`add_annotation_row` | Additional annotations to show for each row in the heatmap. Must be a dataframe with one row for each row in the fData table of cds_subset, with matching IDs.
`add_annotation_col` | Additional annotations to show for each column in the heatmap. Must be a dataframe with one row for each cell in the pData table of cds_subset, with matching IDs.
`show_rownames` | Whether to show the names for each row in the table.
`use_gene_short_name` | Whether to use the short names for each row. If FALSE, uses row IDs from the fData table.
`scale_max` | The maximum value (in standard deviations) to show in the heatmap. Values larger than this are set to the max.
`scale_min` | The minimum value (in standard deviations) to show in the heatmap. Values smaller than this are set to the min.
`norm_method` | Determines how to transform expression values prior to rendering
`trend_formula` | A formula string specifying the model used in fitting the spline curve for each gene/feature.
`return_heatmap` | Whether to return the pheatmap object to the user.
`cores` | Number of cores to use when smoothing the expression curves shown in the heatmap.
`...` | Additional arguments passed to buildBranchCellDataSet

### Value

A list of heatmap_matrix (expression matrix for the branch committment), ph (pheatmap heatmap object), annotation_row (annotation data.frame for the row), annotation_col (annotation data.frame for the column).

[Package monocle version 2.22.0 Index]