plotSignal {plotgardener} | R Documentation

## Plot any kind of signal track data for a single chromosome

### Description

Plot any kind of signal track data for a single chromosome

### Usage

    plotSignal(
        data,
        binSize = NA,
        binCap = TRUE,
        negData = FALSE,
        chrom,
        chromstart = NULL,
        chromend = NULL,
        assembly = "hg38",
        linecolor = "#37a7db",
        fill = NA,
        ymax = 1,
        range = NULL,
        scale = FALSE,
        bg = NA,
        baseline = TRUE,
        baseline.color = "grey",
        baseline.lwd = 1,
        orientation = "h",
        x = NULL,
        y = NULL,
        width = NULL,
        height = NULL,
        just = c("left", "top"),
        default.units = "inches",
        draw = TRUE,
        params = NULL,
        ...
    )

### Arguments

`data` | Data to be plotted as a character value specifying a bigwig file path, a dataframe in BED format, or a GRanges object with metadata column `score`. Either one `data` argument or a list of two can be provided, where the second `data` will be plotted below the x-axis.
`binSize` | A numeric specifying the length of each data bin in basepairs. Default value is `binSize = NA`.
`binCap` | A logical value indicating whether the function will limit the number of data bins to 8,000. Default value is `binCap = TRUE`.
`negData` | A logical value indicating whether the data has both positive and negative scores and the y-axis should be split. Default value is `negData = FALSE`.
`chrom` | Chromosome of region to be plotted, as a string.
`chromstart` | Integer start position on chromosome to be plotted.
`chromend` | Integer end position on chromosome to be plotted.
`assembly` | Default genome assembly as a string or a assembly object. Default value is `assembly = "hg38"`.
`linecolor` | A character value or vector of length 2 specifying the line color(s) outlining the signal track(s). Default value is `linecolor = "#37a7db"`.
`fill` | A character value or vector of length 2 specifying the fill color(s) of the signal track(s). Default value is `fill = NA`.
`ymax` | A numeric specifying the fraction of the max y-value to set as the height of the plot. Default value is `ymax = 1`.
`range` | A numeric vector of length 2 specifying the y-range of data to plot (c(min, max)).
`scale` | A logical value indicating whether to include a data scale label in the top left corner of the plot. Default value is `scale = FALSE`.
`bg` | Character value indicating background color. Default value is `bg = NA`.
`baseline` | Logical value indicating whether to include a baseline along the x-axis. Default value is `baseline = TRUE`.
`baseline.color` | Baseline color. Default value is `baseline.color = "grey"`.
`baseline.lwd` | Baseline line width. Default value is `baseline.lwd = 1`.
`orientation` | A string specifying signal track orientation. Default value is `orientation = "h"`. Options are: `"v"`: Vertical signal track orientation. `"h"`: Horizontal signal track orientation.
`x` | A numeric or unit object specifying signal plot x-location.
`y` | A numeric, unit object, or character containing a "b" combined with a numeric value specifying signal plot y-location. The character value will place the signal plot y relative to the bottom of the most recently plotted plot according to the units of the plotgardener page.
`width` | A numeric or unit object specifying signal plot width.
`height` | A numeric or unit object specifying signal plot height.
`just` | Justification of signal plot relative to its (x, y) location. If there are two values, the first value specifies horizontal justification and the second value specifies vertical justification. Possible string values are: `"left"`, `"right"`, `"centre"`, `"center"`, `"bottom"`, and `"top"`. Default value is `just = c("left", "top")`.
`default.units` | A string indicating the default units to use if `x`, `y`, `width`, or `height` are only given as numerics. Default value is `default.units = "inches"`.
`draw` | A logical value indicating whether graphics output should be produced. Default value `draw = TRUE`.
`params` | An optional pgParams object containing relevant function parameters.
`...` | Additional grid graphical parameters. See gpar.

### Details

#A signal track can be placed on a plotgardener coordinate page by providing plot placement parameters:

    plotSignal(data, chrom,
                chromstart = NULL, chromend = NULL,
                x, y, width, height, just = c("left", "top"),
                default.units = "inches")

This function can also be used to quickly plot an unannotated signal track by ignoring plot placement parameters:

    plotSignal(data, chrom,
                chromstart = NULL, chromend = NULL)

### Value

Returns a `signal` object containing relevant genomic region, placement, and grob information.

### Examples

    ## Load signal data
    library(plotgardenerData)
    data("IMR90_ChIP_H3K27ac_signal")
    data("GM12878_ChIP_H3K27ac_signal")

    ## Create a page
    pageCreate(width = 7.5, height = 2.1, default.units = "inches")

    ## Define region
    region <- pgParams(
        chrom = "chr21",
        chromstart = 28000000, chromend = 30300000,
        assembly = "hg19",
        range = c(0, 45)
    )

    ## Plot and place signal plots
    signal1 <- plotSignal(
        data = IMR90_ChIP_H3K27ac_signal, params = region,
        x = 0.5, y = 0.25, width = 6.5, height = 0.65,
        just = c("left", "top"), default.units = "inches"
    )

    signal2 <- plotSignal(
        data = GM12878_ChIP_H3K27ac_signal, params = region,
        linecolor = "#7ecdbb",
        x = 0.5, y = 1, width = 6.5, height = 0.65,
        just = c("left", "top"), default.units = "inches"
    )

    ## Plot genome label
    plotGenomeLabel(
        chrom = "chr21",
        chromstart = 28000000, chromend = 30300000,
        assembly = "hg19",
        x = 0.5, y = 1.68, length = 6.5,
        default.units = "inches"
    )

    ## Add text labels
    plotText(
        label = "IMR90", fonsize = 10, fontcolor = "#37a7db",
        x = 0.5, y = 0.25, just = c("left", "top"),
        default.units = "inches"
    )
    plotText(
        label = "GM12878", fonsize = 10, fontcolor = "#7ecdbb",
        x = 0.5, y = 1, just = c("left", "top"),
        default.units = "inches"
    )

    ## Hide page guides
    pageGuideHide()

[Package plotgardener version 1.0.17 Index]