read_bam {plyranges} | R Documentation

## Read a BAM file

### Description

Read a BAM file

### Usage

    read_bam(file, index = file, paired = FALSE)

### Arguments

`file` | A connection or path to a BAM file
`index` | The path to the BAM index file
`paired` | Whether to treat alignments as paired end (TRUE) or single end (FALSE). Default is FALSE.

### Details

Reading a BAM file is deferred until an action such as using `summarise()` or `mutate()` occurs. If paired is set to TRUE, when alignments are loaded, the GRanges has two additional columns called read_pair_id and read_pair_group corresponding to paired reads and is grouped by the read_pair_group.

Certain verbs have different behaviour, after using `read_bam()`.

For `select()` valid columns are the fields available in the BAM file. Valid entries are qname (QNAME), flag (FLAG), rname (RNAME), strand, pos (POS), qwidth (width of query), mapq (MAPQ), cigar (CIGAR), mrnm (RNEXT), mpos (PNEXT), isize (TLEN), seq (SEQ), and qual (QUAL). Any two character tags in the BAM file are also valid.

For `filter()` the following fields are valid, to select the FALSE option place `!` in front of the field:

- `is_paired` Select either unpaired (FALSE) or paired (TRUE) reads.

- `is_proper_pair` Select either improperly paired (FALSE) or properly paired (TRUE) reads. This is dependent on the alignment software used.

- 'is_unmapped_query“ Select unmapped (TRUE) or mapped (FALSE) reads.

- `has_unmapped_mate` Select reads with mapped (FALSE) or unmapped (TRUE) mates.

- `is_minus_strand` Select reads aligned to plus (FALSE) or minus (TRUE) strand.

- `is_mate_minus_strand` Select reads where mate is aligned to plus (FALSE) or minus (TRUE) strand.

- `is_first_mate_read` Select reads if they are the first mate (TRUE) or not (FALSE).

- `is_second_mate_read` Select reads if they are the second mate (TRUE) or not (FALSE).

- `is_secondary_alignment` Select reads if their alignment status is secondary (TRUE) or not (FALSE). This might be relevant if there are multimapping reads.

- `is_not_passing_quality_controls` Select reads that either pass quality controls (FALSE) or that do not (TRUE).

- `is_duplicate` Select reads that are unduplicated (FALSE) or duplicated (TRUE). This may represent reads that are PCR or optical duplicates.

### Value

A DeferredGenomicRanges object

### See Also

`Rsamtools::BamFile()`,`GenomicAlignments::readGAlignments()`

### Examples

    if (require(pasillaBamSubset)) {
       bamfile <- untreated1_chr4()
       # nothing is read until an action has been performed
       print(read_bam(bamfile))
       # define a region of interest
       roi <- data.frame(seqnames = "chr4", start = 5e5, end = 7e5) %>%
                as_granges()
       rng <- read_bam(bamfile) %>%
                select(mapq) %>%
                filter_by_overlaps(roi)
    }

[Package plyranges version 1.14.0 Index]