pre_processRDS {scMAGeCK} | R Documentation

## Integrate the imformation of sgRNA into RDS file for the further analysis.

### Description

Pre-process the sgRNA count from previous step, and generate the sgRNA expression matrix.

### Usage

    pre_processRDS(BARCODE, RDS)

### Arguments

`BARCODE` | A txt file to include cell identity information, generated from the cell identity collection step.
`RDS` | A Seurat object or local RDS file path that contains the scRNA-seq dataset. Note that the dataset has to be normalized and scaled.

### Examples

        ### BARCODE file contains cell identity information, generated from the cell identity collection step
        BARCODE <- system.file("extdata","barcode_rec.txt",package = "scMAGeCK")

        ### RDS can be a Seurat object or local RDS file path that contains the scRNA-seq dataset
        RDS <- system.file("extdata","singles_dox_mki67_v3.RDS",package = "scMAGeCK")

        Demo <- pre_processRDS(BARCODE = BARCODE, RDS = RDS)

[Package scMAGeCK version 1.6.0 Index]