kersplatSimLibSizes {splatter} | R Documentation

## Simulate Kersplat library sizes

### Description

Generate library sizes for cells in the Kersplat simulation

### Usage

    kersplatSimLibSizes(sim, params, verbose)

### Arguments

`sim` | SingleCellExperiment containing simulation.
`params` | KersplatParams object with simulation parameters.
`verbose` | logical. Whether to print progress messages

### Details

Library sizes are simulated in one of two ways depending on the value of the `lib.method` parameter.

If `lib.method` is "fit" (default) then means are sampled from a log-normal distribution with meanlog equals `lib.loc` and sdlog equals `lib.scale`.

If `mean.method` is "density" then library sizes are sampled from the density object in the `lib.density` parameter using a rejection sampling method. This approach is more flexible but may violate some statistical assumptions.

Ambient library sizes are also generated from a log-normal distribution based on the parameters for the cell library size and adjusted using the `ambient.scale` parameter.

### Value

SingleCellExperiment with library sizes

[Package splatter version 1.18.2 Index]