associateTranscriptionFactors {MotifDb} | R Documentation

## associateTranscriptionFactors

### Description

In the analysis of, or exploration of gene regulatory networks, one often creates a data.frame of possible genomic regulatory sites, genomic locations where a TF binding motif matches some DNA sequence. A common next step is to associate each of these motifs with its related transcription factor/s. We provide two sources for those relationships. When you specify the "MotifDb" source, we return the motif/TF relationships provided by each of the constituent public MotifDb sources. When you specify the "TFClass" source, transcription factor family memberships (described in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383905/) are - sometimes expansively - provided for each motif you supply.

This method uses, and therefore expects, different columns of the incoming data.frame to be used with each method. The MotifDb source uses the "motifName" column of the incoming data.frame. The TFClass source expects a "shortName" column in the incoming database.

A new column, "geneSymbol", is added to the incoming data.frame. This new column identifies the transcription factor associated with the motif for each row in the data.frame.

### Usage

    ## S4 method for signature 'MotifList'
    associateTranscriptionFactors(object, tbl.withMotifs, source, expand.rows, motifColumnName="motifName")

### Arguments

`object` | a `MotifList` object.
`tbl.withMotifs` | a `data.frame`
`source` | a `character` string, either "MotifDb" or "TFClass" (case insensitive)
`expand.rows` | a `logical` value, recommended especially for the TFClass source, in which sometimes many TFs are mapped to the same motif
`motifColumnName` | a `character` string identifying the column in tbl.withMotifs which contains the motifs to be associated with transcription factors

### Value

A data.frame with one column ("geneSymbol") and possibly multiple rows added

### Author(s)

Paul Shannon

### See Also

MotifDb, geneToMotif, motifToGene, subset, query

### Examples

      tbl.tfClassExample <- data.frame(motifName=c("MA0006.1", "MA0042.2", "MA0043.2"),
                                       chrom=c("chr1", "chr1", "chr1"),
                                       start=c(1000005, 1000085, 1000105),
                                       start=c(1000013, 1000092, 1000123),
                                       score=c(0.85, 0.92, 0.98),
                                       stringsAsFactors=FALSE)
          # here we illustrate how to add a column with the required name:
      tbl.tfClassExample$shortMotif <- tbl.tfClassExample$motifName
      tbl.out <- associateTranscriptionFactors(MotifDb, tbl.tfClassExample, source="TFClass",
                                               expand.rows=TRUE)
      dim(tbl.out)   #    MANY tfs mapped, mostly FOX family genes
      tbl.motifDbExample <- data.frame(motifName=c("Mmusculus-jaspar2016-Ahr::Arnt-MA0006.1",
                                                   "Hsapiens-jaspar2016-FOXI1-MA0042.2",
                                                   "Hsapiens-jaspar2016-HLF-MA0043.2"),
                                        chrom=c("chr1", "chr1", "chr1"),
                                        start=c(1000005, 1000085, 1000105),
                                        start=c(1000013, 1000092, 1000123),
                                        score=c(0.85, 0.92, 0.98),
                                        stringsAsFactors=FALSE)

       tbl.out <- associateTranscriptionFactors(MotifDb, tbl.motifDbExample, source="MotifDb",
                                                expand.rows=TRUE)
       dim(tbl.out)   # one new column ("geneSymbol"), no new rows

[Package MotifDb version 1.24.1 Index]