SplitFile {Sconify} | R Documentation

## Runs "process.multiple.files" on a single file, splits it randomly, and presends half of it is "unstim" and half of it is "stim"

### Description

This is meant to serve as a control for the basic "unstim" and "stim" pipeline that is generally used within this package. If phosphoproteins are being compared across conditions, for example, then there should be no difference in the case that you split the same file and compare the two halves.

### Usage

    SplitFile(file, transform = "asinh", numcells = 10000, norm = FALSE,
      scale = FALSE, input.markers)

### Arguments

`file` | the file we're going to split
`transform` | if set to asinh, performs asinh transformation on all markers of the dataset
`numcells` | the number of total cells to be subsampled to, set at 10k for default
`norm` | boolean of whether data should be quantile normalized
`scale` | boolean of whether data should be z-scored
`input.markers` | vector of strings indicating the markers to be used as input

### Value

tibble containing original markers and all values calculated by SCONE

### Examples

    file <- system.file("extdata", "Bendall_et_al_Cell_Sample_C_basal.fcs",
        package = "Sconify")
    SplitFile(file, input.markers = input.markers)

[Package Sconify version 1.2.1 Index]