writeReport {cellHTS2} | R Documentation

## Create a directory with HTML pages of linked tables and plots documenting the contents of a cellHTS experiment

### Description

Creates a directory with HTML pages of linked tables and plots documenting the contents of the preprocessing of a `cellHTS` object.

### Usage

    writeReport(
      raw,
      normalized=NULL,
      scored=NULL,
      cellHTSlist=NULL,
      outdir,
      force=FALSE,
      map=FALSE,
      plotPlateArgs=NULL,
      imageScreenArgs=NULL,
      posControls,
      negControls,
      mainScriptFile=NA,
      gseaModule=NULL,
      settings=list())

### Arguments

`raw` | the intitial raw `cellHTS` object. See details.
`normalized` | a normalized `cellHTS` object. See details.
`scored` | a scored `cellHTS` object. See details.
`cellHTSlist` | a list of `cellHTS` objects. See details. Note: this argument is deprecated. Please use the separate arguments `raw`, `normalized` and `scored` instead.
`outdir` | a character of length 1 with the name of a directory where to write the report HTML file and images. If the directory does not exist, it is created. If it exists and is not empty, then the behaviour depends on the value of `force`. If `outdir` is missing, it is set to `file.path(getwd(), name(cellHTSlist[['xraw']]))`, i.e. a directory with the name of the cellHTS object(s) in the current working path.
`force` | a logical value, determines the behaviour of the function if `outdir` exists and is not empty. If `force` is `TRUE`, the function overwrites (removes and recreates) `outdir`, otherwise it casts an error.
`map` | a logical value indicating whether tooltips with the annotation should be added to the plate plots and image screen. Default value is FALSE. NOTE: This argument is deprecated and will go away in the next release. Please see `settings` to learn how to control the output of `writeReport`.
`plotPlateArgs` | either a list with parameters for the plate plots of the per plate quality report pages, or a logical scalar with values `FALSE` or `TRUE`. If `FALSE` or `NULL`, the plate plots are omitted, this option is here because the production of the plate plots takes a long time. See details. NOTE: This argument is deprecated and will go away in the next release. Please see `settings` to learn how to control the output of `writeReport`.
`imageScreenArgs` | a list with parameters for the function `imageScreen`. See details. NOTE: This argument is deprecated and will go away in the next release. Please see `settings` to learn how to control the output of `writeReport`.
`posControls` | a list or vector of regular expressions specifying the name of the positive controls. See details.
`negControls` | a vector of regular expressions specifying the name of the negative controls. See details.
`mainScriptFile` | The path to the R script generating the current report. We strongly advice to store this script in the compendium for future reference.
`gseaModule` | Add the output of a gene set enrichment analysis to the report. This is totally experimental at this time.
`settings` | A named list of settings controlling the output of `writeReport`. Please see `settings` for details.

### Details

The function has to be called with the mandatory argument `raw` corresponding to an unnormalized `cellHTS` object (i.e. `state(cellHTSlist[["raw"]])["normalized"]=FALSE`). Additional optional arguments are:

- `"normalized"`: a `cellHTS` object containing normalized data (i.e. `state(cellHTSlist[["normalized"]])["normalized"]=TRUE` and `state(cellHTSlist[["normalized"]])["scored"]=FALSE`).

- `"scored"`: a `cellHTS` object containing data scored data (i.e. `state(cellHTSlist[["scored"]])["scored"]=TRUE`). If this component is available, then `cellHTSlist[["normalized"]]` should also be given.

All of the above arguments have to be `cellHTS` objects containing data from the same experiment, but in different preprocessing stages.

The `cellHTS` argument is deprecated and should no be used anymore.

The following elements are recognized for `plotPlateArgs` and passed on to `plotPlate`: `sdcol`, the color scheme for the standard deviation plate plot, `sdrange`, the sd range to which the colors are mapped, `xcol`, the color scheme for the intensity plate plot, `xrange`, the intensity range to which the colors are mapped. If an element is not specified, default values are used. Both `sdrange` and `xrange` can also be provided as functions, which take the values to be plotted by `platePlot` as a single argument and has to return a numeric vector of length 2. See its documentation for details.

The following elements are recognized for `imageScreenArgs` and passed on to `imageScreen`: `ar`, aspect ratio, `zrange`, range, `anno`, gene annotation for the image map (if `map=TRUE`).

From now on, all settings controlling the output of `writeReport` should either be provided through the `settings` argument, or as session-wide parameters set using `setSettings`. Please see `settings` for details.

`posControls` and `negControls` should be given as a vector of regular expression patterns specifying the name of the positive(s) and negative(s) controls, respectivey, as provided in the plate configuration file (and acccessed via `wellAnno(objects)`).

If the `cellHTS` object containing normalized data was provided as argument `norm`, the length of `posControls` and `negControls` should be equal to the number of channels in this `cellHTS` object (`dim(Data(cellHTSlist[["normalized"]]))[3]`). Otherwise, the length of these vectors should be equal to the number of channels in the unpreprocessed `cellHTS` object (i.e., `dim(Data(cellHTSlist[["raw"]]))[3]`).

By default, if `posControls` is not given, "pos" will be taken as the name for the wells containing positive controls. Similarly, if `negControls` is missing, by default "neg" will be considered as the name used to annotate the negative controls. The content of `posControls` and `negControls` will be passed to `regexpr` for pattern matching within the well annotation given in column `controlStatus` of the `featureData` slot of the `cellHTS` object. If no controls are available for a given channel, use `""` or `NA` for that channel. For example, `posControls = c("", "(?i)^diap$")` means that channel 1 has no positive controls, while "diap" is the positive control for channel 2.

The arguments `posControls` and `negControls` are particularly useful in multi-channel data since the controls might be reporter-specific, or after normalizing multi-channel data.

In the case of a two-way assay, where two types of "positive" controls are used in the screen ("activators" and "inhibitors"), `posControls` should be defined as a list with two components (called `act` and `inh`), each of which should be vectors of regular expressions of the same length as the current number of reporters (as explained above).

By default, tooltips doing the mapping between the probe annotation and the plate wells are not added to the plate plots and to the overall screen plot. If any of the `cellHTS` objects in `cellHTSlist` is annotated, the probe annotation uses the information contained either in column `GeneSymbol` or column `GeneID` (if the former is missing) of the `featureData` slot of the annotated `cellHTS` object. Otherwise, the mapping simply uses the well identifiers.

### Value

The function is called for its side-effect. It returns a character with the full path and name of the report index file, this is an HTML file which can be read by a web browser.

### Author(s)

Florian Hahne florian.hahne@novartis.com, Ligia P. Bras ligia@ebi.ac.uk, Wolfgang Huber huber@ebi.ac.uk, Gabor Bakos

### References

Boutros, M., Bras, L.P. and Huber, W. (2006) Analysis of cell-based RNAi screens, Genome Biology 7, R66.

### See Also

`plotPlate`, `imageScreen`

### Examples

        data(KcViabSmall)
        pCtrls <- c("pos")
        nCtrls <- c("neg")
    ## Not run:
        ## or for safety reasons (not a problem for the current well annotation, however)
         pCtrls <- c("^pos$")
         nCtrls <- c("^neg$")
        writeReport(raw=KcViabSmall, posControls=pCtrls, negControls=nCtrls)
        ## same as
        ## writeReport(raw=KcViabSmall)
        xn <- normalizePlates(KcViabSmall, scale="multiplicative", log=FALSE, method="median")
        xsc <- scoreReplicates(xn, sign="-", method="zscore")
        xsc <- summarizeReplicates(xsc, summary="min")
        ## to turn on the tooltips in the plate plots and in the image screen plot:
        writeReport(raw=KcViabSmall, normalized=xn, scored=xsc, force=TRUE, map=TRUE, plotPlateArgs = TRUE, imageScreenArgs=list(zrange=c(-4,4)))

    ## End(Not run)

[Package cellHTS2 version 2.46.1 Index]