calculate.dNSAF {isobar} | R Documentation

## dNSAF approximate abundance calculations.

### Description

Distributed normalized spectral abundance factor (dNSAF) is a label free quantitative measure of protein abundance based on spectral counts which are corrected for peptides shared by multiple proteins. Original publication: Zhang Y et al., Analytical Chemistry (2010).

### Usage

    calculate.dNSAF(protein.group, use.mw = FALSE, normalize = TRUE,
                    combine.f = mean)

### Arguments

`protein.group` | ProteinGroup object. Its `@proteinInfo` slot `data.frame` must contain a `length` column.
`use.mw` | Use MW to account for protein size
`normalize` | Normalize dSAF to dNSAF?
`combine.f` | How to handle proteins seen only with shared peptides?

### Value

Named numeric vector of dNSAF values.

### Author(s)

Florian P Breitwieser

### References

Zhang Y et al., Analytical Chemistry (2010)

### See Also

`proteinInfo`, `getProteinInfoFromUniprot`, `calculate.emPAI`, `ProteinGroup`

### Examples

    data(ibspiked_set1)
    protein.group <- proteinGroup(ibspiked_set1)
    calculate.dNSAF(protein.group)

[Package isobar version 1.28.1 Index]