networkPlot {piano} | R Documentation

## Gene set network plot

### Description

Draws a network with gene sets as nodes and the thickness of the edges correlating to the number of shared genes. The gene set significance is visualized as color intensities. Gives an overview of the influence of overlap on significant gene sets.

### Usage

    networkPlot(gsaRes, class, direction, adjusted = FALSE,
      significance = 0.001, geneSets = NULL, overlap = 1, lay = 1,
      label = "names", cexLabel = 0.9, ncharLabel = 25, cexLegend = 1,
      nodeSize = c(10, 40), edgeWidth = c(1, 15), edgeColor = NULL,
      scoreColors = NULL, main)

### Arguments

`gsaRes` | an object of class `GSAres`, as returned from `runGSA()` or an object returned from `runGSAhyper()`.
`class` | a character string determining the p-values of which directionality class that should be used as significance information for the plot. Can be one of `"distinct"`, `"mixed"`, `"non"`. Has to be `"non"` if the result from `runGSAhyper()` is used.
`direction` | a character string giving the direction of regulation, can be either `"up"`, `"down"` or `"both"` (for `pValue="distinct"` only).
`adjusted` | a logical, if adjusted p-values should be used, or not. Note that if `runGSA` was run with the argument `adjMethod="none"`, the adjusted p-values will be equal to the original p-values.
`significance` | the significance cut-off that determines which gene sets are included in the plot. Defaults to 0.001.
`geneSets` | a character vector of gene set names, to be included in the plot. Defaults to `NULL`, but if given, the argument `significance` will not be used.
`overlap` | a positive numerical. Determines the smallest number of sharing genes between two gene-sets that is needed in order to draw a line/edge between the gene-sets. Defaults to 1.
`lay` | a numerical between 1-5, or a layout function (see `layout` in the `igraph` package). 1-5 sets the layout to one of the five default layout for the network plot.
`label` | a character string, either `"names"` ,`"numbers"`, `"numbersAndSizes"` or `"namesAndSizes"`, determining the labels used for the nodes. The names are the gene set names, numbers is an arbritary numbered list of the gene sets used in the plot connected to the named list returned by the funtion. Sizes are the gene set sizes, e.g. the number of genes.
`cexLabel` | the text size of the node labels.
`ncharLabel` | the number of characters to include in the node labels.
`cexLegend` | the text size of the legend.
`nodeSize` | a numerical vector of length 2 giving the maximum and minimum node sizes. The node size represents the size of the gene set, and all values will be scaled to the given interval.
`edgeWidth` | a numerical vector of length 2 giving the maximum and minimum edge widths. The edge width represents the number of shared genes between two gene sets, and all values will be scaled to the given interval.
`edgeColor` | a character vector giving the colors to use for increasing edge width. Can also be set to a single color. Defaults to a gray-scale.
`scoreColors` | a character vector giving the colors from which the gradient used for node coloring will be created. In the case of `pValue="distinct"` and `direction="both"` the first half of the vector will be used for the up-regulated gene sets and the second part will be used for the down-regulated gene sets.
`main` | an optional character vector setting the title of the plot.

### Details

In the case of `pValue="distinct"` and `direction="both"`, the distinct directional p-values (`pDistinctDirUp` and `pDistinctDirDn`, see `runGSA`) will be used in combination. Using the `geneSets` and `lay` arguments, multiple comparative plots (i.e. with the same layout) can be drawn, based for instance on the output gene set list from other network plots with different directionality classes.

### Value

Returns a list with two components: `geneSets` containing the names and numbers of the gene sets in the plot, and `layout`, containing the saved layout of the plot, which can be passed back to the `lay` argument in order to draw a subsequent plot with the same layout.

### Author(s)

Leif Varemo piano.rpkg@gmail.com and Intawat Nookaew piano.rpkg@gmail.com

### See Also

piano, `runGSA`, `GSAheatmap`, `layout`

### Examples

       # Load example input data to GSA:
       data("gsa_input")

       # Load gene set collection:
       gsc <- loadGSC(gsa_input$gsc)

       # Run gene set analysis:
       gsares <- runGSA(geneLevelStats=gsa_input$pvals , directions=gsa_input$directions,
                        gsc=gsc, nPerm=500)

       # Network plot:
       networkPlot(gsares,class="non",significance=0.01)

       # Use circular layout and save the layout:
       nw <- networkPlot(gsares,class="non",significance=0.01,lay=5)

       # Use the saved layout to overlay the distinct-directional p-values for easy comparison.
       # Note that the gene sets are now not selected based on a significance cutoff, but from a list:
       networkPlot(gsares,class="distinct",direction="both",lay=nw$layout,geneSets=nw$geneSets)

[Package piano version 1.22.0 Index]