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\documentclass[12pt]{article}

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\begin{document}
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\title{Finding trans eQTL in the Cheung data}
\author{VJ Carey}
\maketitle

<<getl>>=
options(error=recover)
#library(chfive40)
library(cheung2010)
library(hgfocus.db)
library(GenomicRanges)
allst = as.list(hgfocusCHRLOC)
allen = as.list(hgfocusCHRLOCEND)
pchrs = sapply(allst, function(x)names(x)[1])
bad = which(sapply(pchrs,length)==0)
if (length(bad)>0) {
pchrs = pchrs[-bad]
pchrs = sapply(pchrs, function(x)x)
pchrs = paste("chr", pchrs, sep="")
allst = allst[-bad]
allen = allen[-bad]
}
st = sapply(allst, function(x) abs(x)[1])
en = sapply(allen, function(x) abs(x)[1])
gra = GRanges(seqnames=pchrs, IRanges(st,en))
names(gra) = names(allst)
gra = split(gra, seqnames(gra))
lkg = getSS("cheung2010", "chr5")
fn5 = featureNames(lkg)
gra = lapply(gra, function(x)x[ intersect(names(x), fn5) ])

library(snplocsDefault(), character.only=TRUE)
if (!exists("c1s") && file.exists("c1s.rda")) load("c1s.rda")
if (!exists("c1s")) c1s = getSNPlocs("ch1", as.GRanges=TRUE)
if ("ch1" %in% seqlevels(c1s)) seqlevels(c1s) = gsub("ch", "chr", seqlevels(c1s))
if (!file.exists("c1s.rda")) save(c1s,file="c1s.rda")

if (!exists("c2s") && file.exists("c2s.rda")) load("c2s.rda")
if (!exists("c2s")) c2s = getSNPlocs("ch2", as.GRanges=TRUE)
if ("ch2" %in% seqlevels(c2s)) seqlevels(c2s) = gsub("ch", "chr", seqlevels(c2s))
if (!file.exists("c2s.rda")) save(c2s,file="c2s.rda")

if (!exists("c3s") && file.exists("c3s.rda")) load("c3s.rda")
if (!exists("c3s")) c3s = getSNPlocs("ch3", as.GRanges=TRUE)
if ("ch3" %in% seqlevels(c3s)) seqlevels(c3s) = gsub("ch", "chr", seqlevels(c3s))
if (!file.exists("c3s.rda")) save(c3s,file="c3s.rda")

if (!exists("c4s") && file.exists("c4s.rda")) load("c4s.rda")
if (!exists("c4s")) c4s = getSNPlocs("ch4", as.GRanges=TRUE)
if ("ch4" %in% seqlevels(c4s)) seqlevels(c4s) = gsub("ch", "chr", seqlevels(c4s))
if (!file.exists("c4s.rda")) save(c4s,file="c4s.rda")

if (!exists("c17s") && !exists("c17s") && file.exists("c17s.rda")) load("c17s.rda")
if (!exists("c17s")) c17s = getSNPlocs("ch17", as.GRanges=TRUE)
if ("ch17" %in% seqlevels(c17s)) seqlevels(c17s) = gsub("ch", "chr", seqlevels(c17s))
if (!file.exists("c17s.rda")) save(c17s,file="c17s.rda")

if (!exists("c19s") && !exists("c19s") && file.exists("c19s.rda")) load("c19s.rda")
if (!exists("c19s")) c19s = getSNPlocs("ch19", as.GRanges=TRUE)
if ("ch19" %in% seqlevels(c19s)) seqlevels(c19s) = gsub("ch", "chr", seqlevels(c19s))
if (!file.exists("c19s.rda")) save(c19s,file="c19s.rda")

#if ("multicore" %in% installed.packages()[,1]) library(multicore)
#system("rm -rf tsco")
#tr1c = transScores("cheung2010", "chr1", ~sex, snpRanges=c1s, geneRanges=gra[["chr1"]])
#save(tr1c, file="tr1c.rda")
#tr2c = transScores("cheung2010", "chr2", ~sex, snpRanges=c2s, geneRanges=gra[["chr2"]])
#save(tr2c, file="tr2c.rda")
#gc()
#tr3c = transScores("cheung2010", "chr3", ~sex, snpRanges=c3s, geneRanges=gra[["chr3"]])
#save(tr3c, file="tr3c.rda")
#gc()
#tr4c = transScores("cheung2010", "chr4", ~sex, snpRanges=c4s, geneRanges=gra[["chr4"]])
#save(tr4c, file="tr4c.rda")
@
We use the probes on chr17 identified as harboring trans regulators by Cheung.
<<dodo>>=
tempfolder = function ()
{
    z = tempfile()
    system(paste("mkdir", z))
    z
}
obsfold = tempfolder()
permfold = tempfolder()
pr17 = structure(c("209165_at", "203654_s_at", "203367_at", "201508_at", 
"218676_s_at", "208982_at", "202148_s_at", "214552_s_at", "214299_at", 
"219282_s_at"), .Names = c("AATF", "COIL", "DUSP14", "IGFBP4", 
"PCTP", "PECAM1", "PYCR1", "RABEP1", "TOP3A", "TRPV2"))
options(verbose=TRUE)
dropNAs = function (x)
{
    if (!(is(x, "smlSet"))) 
        stop("works only for smlSet instances")
    sml <- x@smlEnv$smList
    maf = snpStats::col.summary(sml[[1]])[, "MAF", drop = FALSE]
    allrs = rownames(maf)
    curok = which(!is.na(maf))
    rm(maf)
    if (length(curok) == 0) 
        stop("dropNAs eliminates all SNP on a chromosome, cannot proceed")
    if (length(curok) != length(allrs)) 
        x@smlEnv$smList[[1]] = x@smlEnv$smList[[1]][, curok]
    rm(allrs)
    x
}

if (!exists("mgrsave")) {
 mgrsave = list()
 for (i in 1:22) {
  cat(i)
  cc17 = dropNAs(getSS("cheung2010", paste("chr", i, sep=""), probesToKeep=pr17))
  mgrsave[[i]] = eqtlTests(cc17, ~sex, targdir=obsfold, 
     runname=paste("cc17", i, sep=""))
  rm(cc17)
  gc()
  }
 save(mgrsave, file="mgrsave.rda")
}
set.seed(12345)
if (!exists("mgrsave_perm")) {
 mgrsave_perm = list()
 for (i in 1:22) {
  cat(i)
  cc17 = dropNAs(getSS("cheung2010", paste("chr", i, sep=""), 
              probesToKeep=pr17, wrapperEndo=permEx))
  mgrsave_perm[[i]] = eqtlTests(cc17, ~sex, targdir=permfold, runname=paste("pcc17", i, sep=""))
  rm(cc17)
  gc()
  }
 save(mgrsave_perm, file="mgrsave_perm.rda")
}
@


\end{document}
