makeTxDbPackage {GenomicFeatures} | R Documentation

## Making a TxDb package from annotations available at the UCSC Genome Browser, biomaRt or from another source.

### Description

A TxDb package is an annotation package containing a TxDb object.

The `makeTxDbPackageFromUCSC` function allows the user to make a TxDb package from transcript annotations available at the UCSC Genome Browser.

The `makeTxDbPackageFromBiomart` function allows the user to do the same thing as `makeTxDbPackageFromUCSC` except that the annotations originate from biomaRt.

Finally, the `makeTxDbPackage` function allows the user to make a TxDb package directly from a TxDb object.

### Usage

      makeTxDbPackageFromUCSC(
        version=,
        maintainer,
        author,
        destDir=".",
        license="Artistic-2.0",
        genome="hg19",
        tablename="knownGene",
        transcript_ids=NULL,
        circ_seqs=DEFAULT_CIRC_SEQS,
        url="http://genome.ucsc.edu/cgi-bin/",
        goldenPath_url="http://hgdownload.cse.ucsc.edu/goldenPath",
        taxonomyId=NA,
        miRBaseBuild=NA)

    makeFDbPackageFromUCSC(
        version,
        maintainer,
        author,
        destDir=".",
        license="Artistic-2.0",
        genome="hg19",
        track="tRNAs",
        tablename="tRNAs",
        columns = UCSCFeatureDbTableSchema(genome, track, tablename),
        url="http://genome.ucsc.edu/cgi-bin/",
        goldenPath_url="http://hgdownload.cse.ucsc.edu/goldenPath",
        chromCol=NULL,
        chromStartCol=NULL,
        chromEndCol=NULL,
        taxonomyId=NA)

      makeTxDbPackageFromBiomart(
        version,
        maintainer,
        author,
        destDir=".",
        license="Artistic-2.0",
        biomart="ENSEMBL_MART_ENSEMBL",
        dataset="hsapiens_gene_ensembl",
        transcript_ids=NULL,
        circ_seqs=DEFAULT_CIRC_SEQS,
        filter=NULL,
        id_prefix="ensembl_",
        host="www.ensembl.org",
        port=80,
        taxonomyId=NA,
        miRBaseBuild=NA)

      makeTxDbPackage(txdb,
                      version,
      		  maintainer,
                      author,
      	          destDir=".",
                      license="Artistic-2.0",
                      pkgname=NULL,
                      provider=NULL,
                      providerVersion=NULL)

      supportedMiRBaseBuildValues()

      makePackageName(txdb)

### Arguments

`version` | What is the version number for this package?
`maintainer` | Who is the package maintainer? (must include email to be valid). Should be a `person` object, or something coercible to one, like a string. May be omitted if the `author` argument is a `person` containing someone with the maintainer role.
`author` | Who is the creator of this package? Should be a `person` object, or something coercible to one, like a character vector of names. The `maintainer` argument will be merged into this list.
`destDir` | A path where the package source should be assembled.
`license` | What is the license (and it's version)
`biomart` | which BioMart database to use. Get the list of all available BioMart databases with the `listMarts` function from the biomaRt package. See the details section below for a list of BioMart databases with compatible transcript annotations.
`dataset` | which dataset from BioMart. For example: `"hsapiens_gene_ensembl"`, `"mmusculus_gene_ensembl"`, `"dmelanogaster_gene_ensembl"`, `"celegans_gene_ensembl"`, `"scerevisiae_gene_ensembl"`, etc in the ensembl database. See the examples section below for how to discover which datasets are available in a given BioMart database.
`genome` | genome abbreviation used by UCSC and obtained by `ucscGenomes()[ , "db"]`. For example: `"hg18"`.
`track` | name of the UCSC track. Use `supportedUCSCFeatureDbTracks` to get the list of available tracks for a particular genome
`tablename` | name of the UCSC table containing the transcript annotations to retrieve. Use the `supportedUCSCtables` utility function to get the list of tables known to work with `makeTxDbFromUCSC`.
`transcript_ids` | optionally, only retrieve transcript annotation data for the specified set of transcript ids. If this is used, then the meta information displayed for the resulting TxDb object will say 'Full dataset: no'. Otherwise it will say 'Full dataset: yes'.
`circ_seqs` | a character vector to list out which chromosomes should be marked as circular.
`filter` | Additional filters to use in the BioMart query. Must be a named list. An example is `filter=as.list(c(source="entrez"))`
`host` | The host URL of the BioMart. Defaults to www.ensembl.org.
`port` | The port to use in the HTTP communication with the host.
`id_prefix` | Specifies the prefix used in BioMart attributes. For example, some BioMarts may have an attribute specified as `"ensembl_transcript_id"` whereas others have the same attribute specified as `"transcript_id"`. Defaults to `"ensembl_"`.
`columns` | a named character vector to list out the names and types of the other columns that the downloaded track should have. Use `UCSCFeatureDbTableSchema` to retrieve this information for a particular table.
`url,goldenPath_url` | use to specify the location of an alternate UCSC Genome Browser.
`chromCol` | If the schema comes back and the 'chrom' column has been labeled something other than 'chrom', use this argument to indicate what that column has been labeled as so we can properly designate it. This could happen (for example) with the knownGene track tables, which has no 'chromStart' or 'chromEnd' columns, but which DOES have columns that could reasonably substitute for these columns under particular circumstances. Therefore we allow these three columns to have arguments so that their definition can be re-specified
`chromStartCol` | Same thing as chromCol, but for renames of 'chromStart'
`chromEndCol` | Same thing as chromCol, but for renames of 'chromEnd'
`txdb` | A TxDb object that represents a handle to a transcript database. This object type is what is returned by `makeTxDbFromUCSC`, `makeTxDbFromUCSC` or `makeTxDb`
`taxonomyId` | By default this value is NA and the organism provided (or inferred) will be used to look up the correct value for this. But you can use this argument to override that and supply your own valid taxId here
`miRBaseBuild` | specify the string for the appropriate build Information from mirbase.db to use for microRNAs. This can be learned by calling `supportedMiRBaseBuildValues`. By default, this value will be set to `NA`, which will inactivate the `microRNAs` accessor.
`pkgname` | By default this value is NULL and does not need to be filled in (a package name will be generated for you). But if you override this value, then the package and it's object will be instead named after this value. Be aware that the standard rules for package names will apply, (so don't include spaces, underscores or dashes)
`provider` | If not given, a default is taken from the 'Data source' field of the metadata table.
`providerVersion` | If not given, a default is taken from one of 'UCSC table', 'BioMart version' or 'Data source' fields of the metadata table.

### Details

`makeTxDbPackageFromUCSC` is a convenience function that calls both the `makeTxDbFromUCSC` and the `makeTxDbPackage` functions. The `makeTxDbPackageFromBiomart` follows a similar pattern and calls the `makeTxDbFromBiomart` and `makeTxDbPackage` functions. `supportedMiRBaseBuildValues` is a convenience function that will list all the possible values for the miRBaseBuild argument. `makePackageName` creates a package name from a TxDb object. This function is also used by OrganismDbi.

### Value

A TxDb object.

### Author(s)

M. Carlson

### See Also

`makeTxDbFromUCSC`, `makeTxDbFromBiomart`, `makeTxDb`, `ucscGenomes`, `DEFAULT_CIRC_SEQS`

### Examples

    ## First consider relevant helper/discovery functions:
    ## Get the list of tables known to work with makeTxDbPackageFromUCSC():
    supportedUCSCtables(genome="hg19")

    ## Can also list all the possible values for the miRBaseBuild argument:
    supportedMiRBaseBuildValues()

    ## Next are examples of actually building a package:
    ## Not run:
    ## Makes a transcript package for Yeast from the ensGene table at UCSC:
    makeTxDbPackageFromUCSC(version="0.01",
                            maintainer="Some One <so@someplace.org>",
                            author="Some One <so@someplace.com>",
                            genome="sacCer2",
                            tablename="ensGene")

    ## Makes a transcript package from Human by using biomaRt and limited to a
    ## small subset of the transcripts.
    transcript_ids <- c(
        "ENST00000400839",
        "ENST00000400840",
        "ENST00000478783",
        "ENST00000435657",
        "ENST00000268655",
        "ENST00000313243",
        "ENST00000341724")

    makeTxDbPackageFromBiomart(version="0.01",
                               maintainer="Some One <so@someplace.org>",
                               author="Some One <so@someplace.com>",
                               transcript_ids=transcript_ids)

    ## End(Not run)

[Package GenomicFeatures version 1.38.2 Index]