steadyState {flowTime} | R Documentation

## Analysis of steady state fluorescence flow cytometry

### Description

Generates a data frame which can be used to visualize and analyze steady state flow cytometry data. Steady state in this case means that

### Usage

    steadyState(flowset, gated = FALSE, ploidy = "diploid", only = "singlets")

### Arguments

`flowset` | your flowSet to be analyzed
`gated` | `boolean` is the data already gated?
`ploidy` | `character` gate to subset your flowset based on the ploidy of you strains
`only` | `character` which population of events to analyze, 'yeast', singlets', or 'doublets'?

### Value

a data frame containing all of the selected subset of events from the original flowSet

### Examples

    dat <- read.flowSet(path = system.file("extdata", "ss_example",
    package = "flowTime"), alter.names = TRUE)
    annotation <- read.csv(system.file("extdata", "ss_example.csv",
    package = "flowTime"))
    dat <- annotateFlowSet(dat, annotation, mergeBy = "name")
    loadGates(gatesFile = 'SORPGates')
    steadyState(dat, gated = FALSE, ploidy = "diploid", only = "singlets")

[Package flowTime version 1.10.0 Index]