extractBFArea {CONFESS} | R Documentation

## extractBFarea

### Description

It estimates the spot or capture site location by BF image modelling.

### Usage

    extractBFArea(cc, img, area, BFarea)

### Arguments

`cc` | Data matrix. An estimated spot center.
`img` | Data matrix. The matrix of image data from a channel.
`area` | Data matrix. The bright (spot) coordinates around the spot center.
`BFarea` | Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated.

### Value

The area identified by BF image modelling

[Package CONFESS version 1.16.0 Index]