PairSummaries {SynExtend} | R Documentation

## Summarize connected pairs in a LinkedPairs object

### Description

Takes in a LinkedPairs object and gene calls, and returns a pairs list.

### Usage

    PairSummaries(SyntenyLinks,
                  GeneCalls,
                  DBPATH,
                  PIDs = TRUE,
                  IgnoreDefaultStringSet = FALSE,
                  Verbose = TRUE,
                  GapPenalty = TRUE,
                  TerminalPenalty = TRUE,
                  Model = "Global",
                  Correction = "none")

### Arguments

`SyntenyLinks` | A `PairedLinks` object.
`GeneCalls` | A list of named DataFrames or GRanges objects. Dataframes built by ”gffToDataFrame” can be used directly, while ”GRanges” objects may also be used with limited functionality. Using a ”GRanges” object will force all alignments to nucleotide alignments.
`DBPATH` | A SQLite connection object or a character string specifying the path to the database file. Constructed from DECIPHER's `Seqs2DB` function.
`PIDs` | Logical indicating whether to perform pairwise alignments. If `TRUE` (the default) all pairs will be aligned using DECIPHER's `AlignSeqs`, or `AlignTranslation` function. This step can be time consuming, especially for large numbers of pairs.
`IgnoreDefaultStringSet` | Logical indicating alignment type preferences. If `FALSE` (the default) pairs that can be aligned in amino acid space will be aligned as an `AAStringSet`. If `TRUE` all pairs will be aligned in nucleotide space.
`Verbose` | Logical indicating whether or not to display a progress bar and print the time difference upon completion.
`GapPenalty` | Argument passed to `AlignTranslation`
`TerminalPenalty` | Argument passed to `AlignTranslation`
`Model` | A character string specifying a model to use to identify pairs that are unlikely to be good orthologs. By default this is ”Global”, but two other models are included; ”Local” and ”Exact”, which have minor differences in performance. Alternatively, a user generated model can be used.
`Correction` | Argument to be passed to `DistanceMatrix`, currently only "none" and "Jukes-Cantor" are supported options. Will only be applied to nucleotide alignments.

### Details

The `LinkedPairs` object generated by `NucleotideOverlap` is a container for raw data that describes possible orthologous relationships, however ultimate assignment of orthology is up to user discretion. `PairSummaries` generates a clear table with relevant statistics for a user to work with as they choose. The option to align all pairs, though onerous can allow users to apply a hard threshold to predictions by PID, while built in models can allow a more succinct and expedient thresholding.

### Value

A data.frame with rownames indicating orthologous pairs.

### Author(s)

Nicholas Cooley npc19@pitt.edu

### See Also

`FindSynteny`, `Synteny-class`

### Examples

    DBPATH <- system.file("extdata",
                          "VignetteSeqs.sqlite",
                          package = "SynExtend")

    # Alternatively, to build a database using DECIPHER:
    # DBPATH <- tempfile()
    # FNAs <- c("ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/006/740/685/GCA_006740685.1_ASM674068v1/GCA_006740685.1_ASM674068v1_genomic.fna.gz",
    #           "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/956/175/GCA_000956175.1_ASM95617v1/GCA_000956175.1_ASM95617v1_genomic.fna.gz",
    #           "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/875/775/GCA_000875775.1_ASM87577v1/GCA_000875775.1_ASM87577v1_genomic.fna.gz")
    # for (m1 in seq_along(FNAs)) {
    #  X <- readDNAStringSet(filepath = FNAs[m1])
    #  X <- X[order(width(X),
    #               decreasing = TRUE)]
    #
    #  Seqs2DB(seqs = X,
    #          type = "XStringSet",
    #          dbFile = DBPATH,
    #          identifier = as.character(m1),
    #          verbose = TRUE)
    #}

    Syn <- FindSynteny(dbFile = DBPATH)

    GeneCalls <- vector(mode = "list",
                        length = ncol(Syn))

    GeneCalls[[1L]] <- gffToDataFrame(GFF = system.file("extdata",
                                                        "GCA_006740685.1_ASM674068v1_genomic.gff.gz",
                                                        package = "SynExtend"),
                                      Verbose = TRUE)
    GeneCalls[[2L]] <- gffToDataFrame(GFF = system.file("extdata",
                                                        "GCA_000956175.1_ASM95617v1_genomic.gff.gz",
                                                        package = "SynExtend"),
                                      Verbose = TRUE)
    GeneCalls[[3L]] <- gffToDataFrame(GFF = system.file("extdata",
                                                        "GCA_000875775.1_ASM87577v1_genomic.gff.gz",
                                                        package = "SynExtend"),
                                      Verbose = TRUE)

    # Alternatively:
    # GeneCalls <- vector(mode = "list",
    #                     length = ncol(Syn))
    # GeneCalls[[1L]] <- rtracklayer::import(system.file("extdata",
    #                                                    "GCA_006740685.1_ASM674068v1_genomic.gff.gz",
    #                                                    package = "SynExtend"))
    # GeneCalls[[2L]] <- rtracklayer::import(system.file("extdata",
    #                                                    "GCA_000956175.1_ASM95617v1_genomic.gff.gz",
    #                                                    package = "SynExtend"))
    # GeneCalls[[3L]] <- rtracklayer::import(system.file("extdata",
    #                                                    "GCA_000875775.1_ASM87577v1_genomic.gff.gz,
    #                                                    package = "SynExtend"))

    names(GeneCalls) <- seq(length(GeneCalls))

    Links <- NucleotideOverlap(SyntenyObject = Syn,
                               GeneCalls = GeneCalls,
                               LimitIndex = FALSE,
                               Verbose = TRUE)

    PredictedPairs <- PairSummaries(SyntenyLinks = Links,
                                    GeneCalls = GeneCalls,
                                    DBPATH = DBPATH,
                                    PIDs = FALSE,
                                    Verbose = TRUE,
                                    Model = "Global",
                                    Correction = "none")

[Package SynExtend version 1.0.0 Index]