gate_quad_tmix {openCyto} | R Documentation

## quadGate based on flowClust::tmixFiler

### Description

This gating method identifies two quadrants (first, and third quadrants) by fitting the data with tmixture model. It is particually useful when the two markers are not well resolved thus the regular quadGate method based on 1d gating will not find the perfect cut points on both dimensions.

### Usage

    gate_quad_tmix(
      fr,
      channels,
      K,
      usePrior = "no",
      prior = list(NA),
      quantile1 = 0.8,
      quantile3 = 0.8,
      trans = 0,
      plot = FALSE,
      ...
    )

### Arguments

`fr` | `flowFrame`
`channels` | `character` vector specifies two channels
`K` | see gate_flowclust_2d
`usePrior` | see gate_flowclust_2d
`prior` | see gate_flowclust_2d
`quantile1` | `numeric` specifies the quantile level(see 'level' in flowClust) for the first quadrant (x-y+)
`quantile3` | `numeric` specifies the quantile level see 'level' in flowClust for third quadrant (x+y-)
`trans` | see gate_flowclust_2d
`plot` | logical whether to plot flowClust clustering results
`...` | other arguments passed to flowClust

### Value

a `filters` object that contains four `polygonGate`s following the order of (-+,++,+-,–)

[Package openCyto version 2.2.0 Index]