shiftCor_dQC {statTarget} | R Documentation

## QC-free based signal correction

### Description

shiftCor_dQC provides the QC-free based signal correction for large scale mass spectrometry-based omics data.

### Usage

    shiftCor_dQC(samPeno, samFile, Frule = 0.8, imputeM = "KNN",
      MLmethod = "Combat", par.prior = TRUE, prior.plots = FALSE,
      mod.covariates = FALSE, batch.Num = NULL)

### Arguments

`samPeno` | The file with the meta information including the sample name, batches, class and order (denoting other covariates besides batch).
`samFile` | The file with the expression information.
`Frule` | Modified n precent rule function. A variable will be kept if it has a non-zero value for at least n precent of samples in any one group. (Default: 0.8)
`imputeM` | The parameter for imputation method i.e., nearest neighbor averaging, 'KNN'; minimum values, 'min'; Half of minimum values, 'minHalf'; median values, 'median'.
`MLmethod` | 'ComBat' allows users to adjust for batch effects in datasets where the batch covariate is known, using methodology described in Johnson et al. 2007. It uses either parametric or non-parametric empirical Bayes frameworks for adjusting data for batch effects. Users are returned an expression matrix that has been corrected for batch effects.The function was revised accroding to 'sva' package (version = "3.8").
`par.prior` | TRUE indicates parametric adjustments will be used, FALSE indicates non-parametric adjustments will be used
`prior.plots` | (Optional) TRUE give prior plots.
`mod.covariates` | TRUE indicates model matrix for outcome of interest and other covariates besides batch (Column 'order' denotes covariates the in samPeno file).
`batch.Num` | (Optional) NULL If given, will use the selected batch as a reference for batch adjustment.

### Value

the shiftCor files. See the details at https://stattarget.github.io

### Examples

    datpath <- system.file('extdata',package = 'statTarget')
    samPeno <- paste(datpath,'MTBLS79_dQC_sampleList.csv', sep='/')
    samFile <- paste(datpath,'MTBLS79.csv', sep='/')
    shiftCor_dQC(samPeno,samFile, Frule = 0.8, MLmethod = "Combat",mod.covariates = FALSE)
    shiftCor_dQC(samPeno,samFile, Frule = 0.8, MLmethod = "Combat",mod.covariates = TRUE,batch.Num = 1)

[Package statTarget version 1.20.0 Index]