## ----knitr, echo = FALSE------------------------------------------------------
library(knitr)
opts_chunk$set(message = FALSE, fig.align = "center", fig.width = 7, fig.height = 7)

## ----netrc_req, echo = FALSE--------------------------------------------------
# This chunk is only useful for BioConductor checks and shouldn't affect any other setup
if (!any(file.exists("~/.netrc", "~/_netrc"))) {
    labkey.netrc.file <- ImmuneSpaceR:::.get_env_netrc()
    labkey.url.base <- ImmuneSpaceR:::.get_env_url()
}

## -----------------------------------------------------------------------------
library(ImmuneSpaceR)
library(data.table)
library(ggplot2)

con <- CreateConnection("SDY144")

## -----------------------------------------------------------------------------
flow <- con$getDataset("fcs_analyzed_result")
hai <- con$getDataset("hai")
vn <- con$getDataset("neut_ab_titer")

## ----subset-------------------------------------------------------------------
pb <- flow[population_name_reported %in% c("Plasma cells,Freq. of,B lym CD27+",
                                           "Plasmablast,Freq. of,Q3: CD19+, CD20-")]
pb <- pb[, population_cell_number := as.numeric(population_cell_number)]
pb <- pb[study_time_collected == 7 & study_time_collected_unit == "Days"] # 13 subjects
pb <- pb[, list(participant_id, population_cell_number, population_name_reported)]

## ----FC-----------------------------------------------------------------------
# HAI
hai <- hai[, response := value_preferred / value_preferred[study_time_collected == 0],
           by = "virus,cohort,participant_id"][study_time_collected == 30]
hai <- hai[, list(participant_id, virus, response)]
dat_hai <- merge(hai, pb, by = "participant_id", allow.cartesian = TRUE)

# VN
vn <- vn[, response:= value_preferred/value_preferred[study_time_collected == 0],
         by = "virus,cohort,participant_id"][study_time_collected == 30]
vn <- vn[, list(participant_id, virus, response)]
dat_vn <- merge(vn, pb, by = "participant_id", allow.cartesian = TRUE)

## ----HAI, dev='png'-----------------------------------------------------------
ggplot(dat_hai, aes(x = population_cell_number, y = response)) +
  geom_point() + 
  geom_smooth(method = "lm") +
  facet_grid(virus ~ population_name_reported, scale = "free") +
  xlab("Number of cells") + 
  ylab("HI fold-increase Day 30 vs. baseline") + 
  theme_IS()

## ----VN, dev='png'------------------------------------------------------------
ggplot(dat_vn, aes(x = population_cell_number, y = response)) +
  geom_point() + 
  geom_smooth(method = "lm") +
  facet_grid(virus ~ population_name_reported, scale = "free") +
  xlab("Number of cells") + 
  ylab("VN fold-increase Day 30 vs. baseline") + 
  theme_IS()

