SimpleCOMPASS {COMPASS} | R Documentation

## Fit the discrete COMPASS Model

### Description

This function fits the `COMPASS` model from a user-provided set of stimulated / unstimulated matrices. See the NOTE for important details.

### Usage

    SimpleCOMPASS(n_s, n_u, meta, individual_id, iterations = 10000,
      replications = 8, verbose = TRUE)

### Arguments

`n_s` | The cell counts for stimulated cells.
`n_u` | The cell counts for unstimulated cells.
`meta` | A `data.frame` of metadata, describing the individuals in the experiment. Each row in `meta` should correspond to a row in `data`. There should be one row for each subject; i.e., one row for each element of `n_s` and `n_u`.
`individual_id` | The name of the vector in `meta` that denotes the individuals from which samples were drawn.
`iterations` | The number of iterations (per 'replication') to perform.
`replications` | The number of 'replications' to perform. In order to conserve memory, we only keep the model estimates from the last replication.
`verbose` | Boolean; if `TRUE` we output progress information.

### Value

A `list` with class `COMPASSResult` with two components, the `fit` containing parameter estimates and parameter acceptance rates, and `data` containing the generated data used as input for the model.

### Note

n_s and n_u counts matrices should contain ALL 2^M possible combinations of markers, even if they are 0 for some combinations. The code expects the marker combinations to be named in the following way: `"M1&M2&!M3"` means the combination represents cells expressing marker "M1" and "M2" and not "M3". For 3 markers, there should be 8 such combinations, such that n_s and n_u have 8 columns.

### Examples

     set.seed(123)
    n <- 10 ## number of subjects
    k <- 3 ## number of markers

    ## generate some sample data
    iid_vec <- paste0("iid_", 1:n) # Subject id
    data <- replicate(2*n, {
    nrow <- round(runif(1) * 1E4 + 1000)
    ncol <- k
    vals <- rexp( nrow * ncol, runif(1, 1E-5, 1E-3) )
    vals[ vals < 2000 ] <- 0
    output <- matrix(vals, nrow, ncol)
    output <- output[ apply(output, 1, sum) > 0, ]
    colnames(output) <- paste0("M", 1:k)
    return(output)
    })

    meta <- cbind(iid=iid_vec, data.frame(trt=rep( c("Control", "Treatment"), each=n/2 )))

    ## generate counts for n_s, n_u
    n_s <- CellCounts( data[1:n], Combinations(k) )
    n_u <- CellCounts( data[(n+1):(2*n)], Combinations(k) )
    rownames(n_s) = unique(meta$iid)
    rownames(n_u) = rownames(n_s)
    ## A smaller number of iterations is used here for running speed;
    ## prefer using more iterations for a real fit
    scr = SimpleCOMPASS(n_s, n_u, meta, "iid", iterations=1000)

[Package COMPASS version 1.16.2 Index]