positiveFactor {NanoStringDiff} | R Documentation

## Acessor functions for the 'positiveFactor' slot in a NanoStringSet object.

### Description

Positive size factors can be used to adjust all platform associated sources of variation.

### Usage

    ## S4 method for signature 'NanoStringSet'
    positiveFactor(object)
    ## S4 replacement method for signature 'NanoStringSet,numeric'
    positiveFactor(object) <- value

### Arguments

`object` | A NanoStringSet object.
`value` | A vector of positive size factors.

### Details

The observed counts including negative control genes and housekeeping control genes might be effect by some experimental factors like hybridization and binding efficiency. In order to get the true rate of gene expression, these variations must be normalized. Positive size factors can normalize this kind of variation.

### Value

A vector contain positive size factors

### Author(s)

Hong Wang <hong.wang@uky.edu> chi wang <chi.wang@uky.edu>

### See Also

positiveControl

### Examples

    data(NanoStringData)
    ## obtain positive factors
    positiveFactor(NanoStringData)

    ## assign a vector
    n=ncol(exprs(NanoStringData))
    positiveFactor(NanoStringData)=rep(1,n)

[Package NanoStringDiff version 1.9.2 Index]